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KMID : 1134820000290040669
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Journal of the Korean Society of Food Science and Nutrition
2000 Volume.29 No. 4 p.669 ~ p.675
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The Effect of Puerariae thubergiana Bentham Extract on Brain Tissue in Alcohol - Treated Rats
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Kim Myung-Joo
Cho Soo-Yeul
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Abstract
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This study investigated the effect of Puerariae Flos (PF: flower of Puerariae plant) and Puerariae Radix (PR: root of Puerariae plant) water extracts on the activities of ethanol-metabolizing enzymes and free radical generating/scavenging enzymes of brain in ethanol-treated rats. Five groups of male Sprague- Dawley rats were orally administered ethanol (25%, v/v) 5 g/kg body weight/day, and sacrificed 5 weeks post treatment. PF and PR water extracts were supplemented in a diet based on 1.2 g (I) or 2.4 g (Ⅱ) raw PF or PR/kg body weight/day. Alcohol dehydrogenase activity of brain was significantly lowered in PF or PR groups, whereas aldehyde dehydrogenase activity was significantly higher in PR groups than those of control and PF groups. Cytochrome P-450 content, aminopyrine D-methylase and aniline hydroxylase activities were decreased in both PF and PR groups compared to control group. Aldehyde oxidase and xanthine oxidase activities tended to decrease by Puerariae plant extract supplemented groups and degree of decrease predominated in PRI. Superoxide dismutase and glutathione S-transferase activities were increased in PF or PR groups, whereas glutathione peroxidase and catalase activities were significantly decreased by Puerariae plant extracts supplement. These results indicated that supplementation of PF or PR lowers free radical generating enzymes activities. It was suggested that the activities of ethanol metabolizing emzymes and antioxidant enzymes in brain can be enhanced by PF or PR supplement in ethanol-treated rats.
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KEYWORD
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Puerariae Flos, Puerariae Radix, ethanol metabolism, brain
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